Medical equipment helicobacter pylori test system detector C13 C14 Urea breath test h.pylori tester

Helicobacter pylori (Hp) is one of the most common bacterial pathogens, and more than half of the world’s population has been infected with Helicobacter pylori. Hp infection is related to a variety of gastrointestinal diseases, such as chronic gastritis, duodenal ulcer, gastric ulcer, non-ulcer dyspepsia, gastric cancer and gastric mucosal-associated lymphoma, and even parenteral diseases. Early diagnosis and treatment of Hp infection is particularly important. The clinical detection methods of Hp infection are mainly divided into two types: invasive and non-invasive. Invasive testing is somewhat traumatic, with poor coagulation function and poor compliance in patients or children taking anticoagulant drugs; non-invasive testing methods are less invasive and make up for the lack of poor compliance in invasive testing.

Methods Comparison:

1. Invasive HP infection detection methodAll invasive Hp detection methods require a gastroscopic biopsy in advance, and subsequent analysis after sampling, mainly as follows:1.1 Separate culture and smearPrinciple: Take biopsy specimens and homogenize them, then use culture medium to streak culture. The morphology of the slides was Gram-negative, S-curved, or Brevibacterium, with typical drilling movement under darkfield or phase-contrast microscope. This method is considered the gold standard and is the definitive evidence of Hp infection. However, this method is time-consuming, laborious, and has a long cycle.1.2 Histological stainingPrinciple: Use conventional pathological sections for staining. Under the oil microscope, Hp showed a typical S-shaped or seagull-shaped curve, located on the surface of the mucosal layer or invading the gastric gland or at the junction of epithelial cells. According to the location and staining and general bacteria identification. This method is one of the gold standards for Hp diagnosis.1.3 Rapid urease test methodPrinciple: Hp is a bacterium that produces a large amount of urease in the human stomach, so Hp infection can be diagnosed by detecting urease. Take a piece of mucosa and gastric antrum mucosa tissue from the lesion and use a rapid urease diagnostic kit for detection. *The final color is yellow if it is negative, rose or light red is considered positive.2. Non-invasive HP infection detection methodThe non-invasive HP detection method does not require gastroscopy and is easy to be accepted. There are mainly the following:2.1 C13/C14 breath testPrinciple: After the subject takes urea C13/C14 capsules orally, if there are Helicobacter pylori in the stomach, the urease produced by it can quickly decompose urea into carbon dioxide and ammonia. The carbon dioxide enters the lungs through the blood and is excreted from the body. After the CO2 gas with the marker is collected and measured on the instrument, it can be judged whether there are Helicobacter pylori in the stomach. Clinically, this method is often used as the "gold standard" for diagnosis, and it is widely used.

Specifications:

Test Ways:

Warm-up after power-onInstall the instrument in a suitable working environment and connect to power. Turn on the power switch of the CHL-HPC14 When it reaches a stable state after warming up for 30 minutes, it can be normally used. Calibrationa. Insert the attached standard background card into the measuring slot of the instrument to calibrate the background for 1000 seconds without inserting a card. The calibration result shall be BGC ≤ BGM. Insert the attached standard background card into the measuring slot for sample measurement (set the parameter MOD_CON to 0), and the measurement result C should be negative. b. Insert the attached standard efficiency card into the measuring slot for sample measurement (set the parameter MOD_CON to 0), and the measurement result C should be within ±30% of the labeled value. When the conditions of a and b are met simultaneously, the instrument can be operated normally. Sample collection:a. The subject takes one 14C-Urea on an empty stomach or two hours after eating. b. Sit for 15-25 minutes. c. Unpack the collection card and connect the mouthpiece to the card body. The subject blows into the card through the mouthpiece with appropriate force. The subject may exchange breath during blowing, but DO NOT draw in a breath! Stop blowing when the card indicator turns from orange to yellow (about 1-3 minutes) or after 3 minutes even though the color is not completely changed. d. Fill in the information of the subject on the collection card after sample breath is collected. Sample testing:Insert the sample card into a measuring slot, and the test will start automatically. The test result will be displayed on the screen when the test completes. Result printing:The test reports can be printed either from an external printer or a built-in micro printer.

pecification

Working procedure

*Should be fasted for 4 to 6 hours before the test * Take about 120ml warm drinking water with urea 14C capsule, wait for 10-20 minutes. * Collect the sample. * Test the sample.

one-time exhalation sample capture card urea carbon -14 capsules